Two Trypsin Inhibitors from Porcine Pancreatic Juice
نویسندگان
چکیده
منابع مشابه
Two trypsin inhibitors from porcine pancreatic juice.
Two trypsin inhibitors of the Kazal type have been isolated from porcine pancreatic juice. They have been prepared in 65% yield by gel titration on Sephadex G-75 at pH 8.1 in the presence of lo-*M diisopropyl phosphofluoridate, tiltration through diethylaminoethyl cellulose at pH 9, and equilibrium chromatography on sulfoethyl Sephadex at pH 5.4. The major component, Porcine Inhibitor I, has a ...
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A trypsin inhibitor has been isolated in 54% yield from bovine pancreatic juice by gel filtration on Sephadex G-75 at pH 8.1 and by elution chromatography on DEAE-cellulose at pH 9.0. It appears to be homogeneous by equilibrium chromatography, equilibrium sedimentation ultracentrifugation, and amino acid analysis, and on the basis of the stoichiometry of its interaction with trypsin. The polype...
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Human pancreatic secretory trypsin inhibitor has been isolated in 55% yield from human pancreatic juice and in 45% yield from human post mortem pancreata by gel filtration on Sephadex G-75 in the presence of 1OW M diisopropyl phosphofluoridate and by ion exchange chromatography on DEAEcellulose and SP-Sephadex. The polypeptide inhibitor has a molecular weight of 6242 and contains 56 amino acid ...
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We studied the distribution of pancreatic secretory trypsin inhibitor (PSTI) in the epithelia of the gastrointestinal tract and determined whether PSTI is secreted into gastric juice. PSTI was measured by a specific radioimmunoassay in biopsy specimens taken from the upper (n = 8) and lower (n = 7) gastrointestinal tract of patients with normal endoscopies. PSTI was present in the stomach, smal...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1968
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)93514-3